Agares

Agar Diclor�n Rosa de Bengala con Cloranfenicol (DRBC): composici�n en g/L
Glucose 10 Peptone 5 Monopotassium Phosphate 1 Magnesium Sulfate 0.5 Chloramphenicol 0.1 Bengal Rose 0.025 Dichloran 0.002 Bacteriological Agar 15 Suspend 31.6 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121�C for 15 minutes. Cool to 45-50�C, mix well and dispense into plates. The prepared medium should be stored at 8-15�C.

Agar Diclor�n Rosa de Bengala con Cloranfenicol (DRBC): composici�n en g/L

Glucose 10
Peptone 5
Monopotassium Phosphate 1
Magnesium Sulfate 0.5
Chloramphenicol 0.1
Bengal Rose 0.025
Dichloran 0.002
Bacteriological Agar 15

Suspend 31.6 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121�C for 15 minutes. Cool to 45-50�C, mix well and dispense into plates. The prepared medium should be stored at 8-15�C.

Agar Diclor�n 18% Glicerol (DG18): composici�n en g/L
Peptone..................................................................................... 5 g Glucose................................................................................... 10 g Monopotassium Phosphate ...................................................... 1 g Magnesium Sulfate ................................................................ 0.5 g Zinc Sulfate .......................................................................... 0.01 g Copper Sulfate ................................................................... 0.005 g Dichloran............................................................................ 0.002 g Chloramphenicol .................................................................. 0.05 g Chlortetracycline .................................................................. 0.05 g Agar ........................................................................................ 15 g Final pH: 5.6 � 0.2 at 25 �C Suspend 31.6 g of the medium and 220 g of glycerol in one liter of purified water. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Autoclave at 121 �C for 15 minutes. DO NOT OVERHEAT.

Agar Diclor�n 18% Glicerol (DG18): composici�n en g/L

Peptone..................................................................................... 5 g
Glucose................................................................................... 10 g
Monopotassium Phosphate ...................................................... 1 g
Magnesium Sulfate ................................................................ 0.5 g
Zinc Sulfate .......................................................................... 0.01 g
Copper Sulfate ................................................................... 0.005 g
Dichloran............................................................................ 0.002 g
Chloramphenicol .................................................................. 0.05 g
Chlortetracycline .................................................................. 0.05 g
Agar ........................................................................................ 15 g
Final pH: 5.6 � 0.2 at 25 �C

Suspend 31.6 g of the medium and 220 g of glycerol in one liter of purified water. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Autoclave at 121 �C for 15 minutes. DO NOT OVERHEAT.